Strengthening case for natural origin.

 

(Image credit: BROKER/Franz Christoph Robiller via Getty Images)

Researchers have discovered coronaviruses lurking in Laotian bats that appear to be the closest known relatives to SARS-CoV-2, the virus that causes COVID-19, found to date, according to news reports.

In a new study, researchers from the Pasteur Institute in France and the University of Laos captured 645 bats from limestone caves in northern Laos and screened them for viruses related to SARS-CoV-2. They found three viruses — which they dubbed BANAL-52, BANAL-103 and BANAL-236 — that infected horseshoe bats and shared more than 95% of their overall genome with SARS-CoV-2.

One of the viruses, BANAL-52, was 96.8% identical to SARS-CoV-2, according to Nature News. That makes BANAL-52 more genetically similar to SARS-CoV-2 than any other known virus. Previously, the closest known relative to SARS-CoV-2 was RaTG13, which was found in horseshoe bats in 2013 and shares 96.1% of its genome with SARS-CoV-2, Nature News reported.

What's more, all three of the newly discovered viruses are more similar to SARS-CoV-2 in a key part of their genome — called the receptor binding domain (RBD) — than other known viruses. The RBD is the part of the virus that allows it to bind to host cells. With SARS-CoV-2, the RBD binds to a receptor known as ACE2 on human cells, and the virus uses this receptor as a gateway into cells.

Critically, the new study found that BANAL-52, BANAL-103 and BANAL-236 can bind to ACE2 and use it to enter human cells. So far, other candidates proposed as ancestors of SARS-CoV-2 found in bats, including RaTG13, haven't been able to do this, the researchers said. The three viruses could bind to ACE2 about as well as early strains of SARS-CoV-2 found in Wuhan, they said.

The findings, which were posted to the preprint server Research Square on Sept. 17, add to the evidence that SARS-CoV-2 had a natural origin, rather than escaping from a lab.

The results show "that sequences very close to those of the early strains of SARS-CoV-2 ... exist in nature," the researchers wrote in their paper, which has yet to be peer-reviewed.

"The receptor binding domain of SARS-CoV-2 looked unusual when it was first discovered because there were so few viruses to compare it to," Edward Holmes, an evolutionary biologist at the University of Sydney, who wasn't involved in the research, told Bloomberg. "Now that we are sampling more from nature, we are starting to find these closely related bits of gene sequence," Holmes said.

The authors say their findings support the hypothesis that SARS-CoV-2 resulted from a recombination of viral sequences existing in horseshoe bats.

Still, even though the newly discovered viruses are closely related to SARS-CoV-2, all three viruses lack a sequence for what is known as the "furin cleavage site," which is seen in SARS-CoV-2 and aids the virus's entry into cells, according to Nature News. This means that in order to better understand the origins of SARS-CoV-2 further research is needed to show how and when the furin site was introduced.

The findings are currently being considered for publication in a Nature journal, Bloomberg reported.

Source:  https://www.livescience.com/closest-bat-virus-found-to-sars-cov2-covid  (Originally published on Live Science.) 

 

We asked the experts how to increase lung capacity so that you can breathe easier during exercise.

Whether you’re reaching the summit of a mountain or coming to the end of a HIIT class, most of us will feel puffed out during exercise. You might have even wondered how to increase lung capacity so that you can keep going during particularly difficult sets.

It’s possible to tone your muscles and sculpt your body, but can you give your lungs an upgrade too? Science has proved that not smoking and doing regular exercise will help keep your lungs healthy and strong. However, will doing a HIIT class or powering through a treadmill workout at the gym help you increase your lung capacity? Live Science asked the experts if the feat is possible.
 

HOW TO INCREASE LUNG CAPACITY

Lungs are spongy, air-filled organs that lie on either side of your chest. They take oxygen from the atmosphere, pass it into your bloodstream and then expel the waste gas (carbon dioxide) that the body doesn’t need.

However, the amount of air that a pair of lungs can contain actually differs from person to person, pulmonologist Dr. Andrew Martin of the Deborah Heart and Lung Center in New Jersey told Live Science. "Lung capacity changes with age and height, so there is no one normal number for lung capacity," said Martin. "Normal lung capacity should be enough to bring oxygen to the blood and eliminate carbon dioxide from it. You should be able to increase your breathing enough so that, even with heavy exercise, you should only be using about 70% of your maximum voluntary ventilation."

Experts can measure your normal lung capacity in several ways, said Dr. Martin. This includes FEV1, where the amount of air you can exhale forcefully in one second is measured; Vital Capacity, which is the amount you can exhale after breathing all the way in and Maximum Voluntary Ventilation (MVV), the maximum amount of air you can move and out of your lungs by breathing in and out as fast as you can over 15 seconds.  

Researchers from the University of Belgrade studied which sports improve lung function the most, finding that basketball players, water polo players and rowers scored the highest in FEV1 and MVV tests. This led them to believe that the training used in these sports could have the potential for improving pulmonary function and rehabilitation.

Lung capacity doesn’t just rely on the lungs alone, as your chest wall and the muscles of respiration, which include the diaphragm and the intercostal muscles (the muscles that lie between your ribs) also play a part.

"A certain amount of increased lung capacity can be gained from physical exercise that involves heavy or deep breathing," said Dr. Martin. "However, the benefits of exercise come mostly from improvements in muscle function, blood flow, and cardiac function."

If you feel you've been a little more sedentary than usual, Dr. Martin said that deep breath holds can open small airways that have become narrowed. "When areas of your lung have become compressed during periods of inactivity, taking a very deep breath and holding it for a few seconds can improve your overall lung capacity. Either sitting up very straight with arms up or standing leaning forward a little with your hands on a table or counter can help with this maneuver."

Most people have more than enough maximum lung capacity even during very vigorous exercise. Dr. Martin said: "In very elite athletes, people with neuromuscular weakness, and in people with severe lung disease, maximum lung function can become a limiting factor and exercises that train the respiratory muscles may offer some benefit."

A few deep breath holds can help "prime" the lungs and make sure all the units are open and functioning at their optimal capacity. "This can be useful before exercise, but one must be careful not to hyperventilate," said Dr. Martin. However, this hyperinflation doesn’t work for everyone. "If airway narrowing is a problem as in an asthma attack, air can get trapped in the lung. This 'hyperinflation' can actually impair lung function."

HOW LUNG FUNCTION IS MEASURED

To measure lung function accurately requires specialized equipment not available to most people at home. Dr. Frank Coletta of Mount Sinai South Nassau hospital, New York told Live Science that while these tests can be used for people suffering from asthma or emphysema, they are also used by sports medicine clinicians caring for elite athletes. The tests include Spirometry, the Dilution Method and Body Plethysmography. 

"They are used to assess and determine the extent of exercise-induced asthma, which is common amongst athletes," said Dr. Coletta. "They are also used to assess symptomatology such as shortness of breath which may be out of proportion to the amount of exercise performed. And they are used in longitudinal studies to assess the impact of training programs and diet."

According to Dr. Coletta, Spirometry is the most basic type of pulmonary function test. "It’s done by having the patient blow into a tube connected to a machine," he explains. "It measures how easily and how fast air can move out of your lungs.  It can also give a measurement of how much air, or capacity, the lungs have."  

The Dilution Method incorporates the above-mentioned Spirometry as well as other maneuvers to get a picture of someone’s complete lung function. "The dilution technique helps us understand how much volume of air is left in the lungs after a maximal expiration," said Dr. Coletta. "In other words, when the patient blows all of the air out of their lungs, there is still a significant amount of air left in the lungs." An inert gas is inhaled by the patient and calculations are made by the computer to assess the complete volume of the lung. 

Body Plethysmography is an alternative way to determine lung capacities, said Dr. Coletta. "The patient is placed inside a sealed glass chamber with a known fixed volume," he said. "The patient is asked to inhale deeply and exhale maximally and changes in pressure within the box are measured."

If you want to remain in tip-top shape, the American College of Sports Medicine recommends 150 minutes of moderate-intensity aerobic exercise each week.

Performance coach Chelsea Chicantek for fitness app Future told Live Science that if you want to feel less winded during activity and want to become more efficient at transporting oxygen around your body try interval training. "Interval training is a great way to improve VO2 max (maximum rate of oxygen used by your body during exercise). In this type of exercise, the participant alternates between intense and low-effort activity," said Chicantek. This idea is also backed up by research from the National Library of Medicine.

No matter whether you have a specific fitness goal in mind, or you simply want to experiment with increasing your lung capacity, it’s always important to consult with your doctor before beginning an exercise program. (Written by Claire Turrell)

Source: https://www.livescience.com/how-to-increase-lung-capacity 

 

On September 21, 2021, the Philippine Eye Research Institute (PERI), in collaboration with the Queen's University of Belfast of the United Kingdom (UK), successfully deployed a validated artificial intelligence (AI) algorithm for diabetic retinopathy screening, marking the first use of AI in ophthalmology in the Philippines.

This is a significant milestone in Philippine ophthalmology and is a step towards establishing an inclusive program for diabetic retinopathy screening that has the potential to eliminate diabetes-related blindness. The UK-Philippines Remote Retinal Evaluation Collaboration in Health: Diabetic Retinopathy or REACH-DR is a Newton-Agham program that aims to establish a diabetic retinopathy screening program (DRSP) here in the country. Establishing a local DRSP will help in the timely identification of eyes at risk for diabetes-related blindness and visual loss. 


To achieve this, REACH-DR pursues the following targets: 1) the development of the necessary infrastructure for a local DRSP by analyzing and validating existing telemedicine technology, 2) adaptation of the selected technologies into the Philippine setting, and 3) completion of technology transfer to the Philippine stakeholders. 


If we succeed in establishing a DRSP locally, we can help treat patients on time, save their eyesight, and help them remain socially active,” project leader and Harvard Medical School Assistant Professor of Ophthalmology Dr. Paolo Antonio Silva says. “Ultimately, we want to reduce the incidence of blindness and visual impairment caused by diabetic retinopathy, which will allow the patients to live independently,” he adds.


We, at the DOST-PCHRD, are proud of the achievement that the REACH-DR team has achieved,” DOST-PCHRD Executive Director Dr. Jaime C. Montoya says. “This project is an example of how we build on research to make healthcare services more accessible for the Filipino people, and ultimately, help make their lives better,” he adds. 


Currently, the REACH-DR team is conducting retinal screening among underserved communities in the National Capital Region (NCR) and Region 3. The screening is expected to be completed by June 2022.

The project is funded under the Newton Agham Program of the DOST-PCHRD and the UK Medical Research Council.

 

Source:https://www.pchrd.dost.gov.ph/news/6712-dost-pchrd-funded-study-marks-the-first-use-of-ai-in-ph-clinical-setting-for-ophthalmology 

Depicted in films, books, and science fiction stories as a means to bring doom to humans, Artificial Intelligence (AI) actually can significantly change medicine and healthcare.

In a report, the World Health Organization (WHO) describes how leveraging AI for healthcare holds great promise or potential. This potential is exactly what Harvard Medical School Assistant Professor of Ophthalmology Dr. Paolo Antonio Silva and his team are exploring with their current project on diabetic retinopathy.


Under the Newton Agham Program of the Department of Science and Technology - Philippine Council for Health Research and Development (DOST-PCHRD) and the United Kingdom Medical Research Council (UK-MRC), Dr. Silva’s team launched the UK-Philippines Remote Retinal Evaluation Collaboration in Health: Diabetic Retinopathy or REACH-DR.

On September 21, 2021, the REACH-DR team shared the first successful implementation of a validated AI algorithm in the clinical setting for ophthalmology in the country. Following this significant achievement, Dr. Silva shares their motivation and vision in pursuing the project, and their plans moving forward.


Establishing a telemedicine program for diabetic retinopathy

Building on AI technologies, the REACH-DR project aims to establish the very first inclusive telemedicine screening program for diabetic retinopathy in the country.

"Diabetic retinopathy is the most common complication of diabetes. Despite the availability of highly effective treatment, diabetic retinopathy remains the most common cause of visual loss and blindness among people with diabetes. This emphasizes the need for retinal evaluation.” Dr. Silva explains. “While screening for the disease early on is especially relevant, in-person retinal evaluation in the Philippines is not always possible, due to lack of access to medical facilities that is worsened by geographic, social and cultural constraints.”

Deriving inspiration from the success of a DR screening program in the UK, Dr. Silva’s team set out to launch a similar project here in 2019. “One of the greatest success stories in diabetes eye care is the United Kingdom's National Diabetic Retinopathy Screening Programme. In 2014, for the first time in five decades, diabetic retinopathy was no longer the leading cause of legal blindness in the working-age population in England and Wales, due to the early detection of diabetic retinopathy by screening, along with improved control of blood sugar levels.”

The team aims to implement a national DRSP in the country by completing three phases: 1) developing the necessary infrastructure by analyzing and validating existing telemedicine technology, 2) adapting the selected technologies into the Philippine setting, and 3) completing the technology transfer to the Philippine stakeholders.

If successful, “the UK REACH DR program will help to identify eyes at high risk for losing sight, and this will have a direct benefit for individuals,” Dr. Silva says. “An additional benefit will be the promotion of awareness for the need for eye evaluations which will significantly improve the overall level of eye care and reduce the risk of diabetes-related visual loss among people with diabetes,” he added.


The first validated AI algorithm in the clinical setting for ophthalmology in PH


Now in its third year of implementation, the project achieved a significant milestone last week. “September 21, 2021, marks the first use of a validated artificial intelligence (AI) algorithm in a clinical setting for ophthalmology in the Philippines,” Dr. Silva shares. “Our REACH-DR team with the use of AI has successfully performed and completed diabetic retinopathy screening in a target community in Nueva Ecija to identify diabetic retinopathy and other vision-threatening retinal diseases.”

 




This development is a step closer to increasing access to retinal screening, which will pave the way for timely and accurate diabetic retinopathy detection.

“Evaluating retinal images is a highly skilled process, which requires training, continuous quality control, and maintenance of a specialized skill set. As trained retinal image readers are costly and difficult to train with limited numbers worldwide, it has become a necessity to seek automation processes in ocular telemedicine to increase throughput while maintaining cost-effectiveness and accuracy.”

The project will run until next year, June 2022, with the completion of the screening of the target population and is expected to provide the framework for the future implementation of diabetic retinopathy screening programs in the Philippines.

With the nearing completion of the project, Dr. Silva highlights how conducting health research provides “opportunities to answer dilemmas unaddressed for many years.”

“Health research changes lives and makes the world we live in much better than it is,
” he says.
__________________________________________________________________________________________________

Dr. Paolo Antonio S. Silva is a staff ophthalmologist and the chief of telemedicine at the Beetham Eye Institute of the Joslin Diabetes Center. He is an Assistant Professor of Ophthalmology at Harvard Medical School and a research collaborator and faculty at the Philippine Eye Research Institute.

Source: https://www.pchrd.dost.gov.ph/news/6713-making-screening-for-diabetic-retinopathy-closer-to-filipino-patients-reach 

The Department of Science and Technology (DOST) and the University of the Philippines (UP) will inaugurate the Protein, Proteomics, and Metabolomics Facility (PPMF) as an addition to the existing genomic research capacities of the UP Philippine Genome Center (PGC) this October 4, 2021.

 

Initiated in 2018, the DOST provided a total of 175 million pesos of funding support for the provision of globally recognized tools and equipment units needed for the establishment of the PPMF. According to DOST Undersecretary for R&D Dr. Rowena Cristina Guevara, the PPMF is envisioned “as a platform for collaborative work on proteomics and metabolomics research and a training hub for those who are conducting OMICS-type researches”

 

With UP’s support on infrastructure development and DOST’s support in the acquisition of several laboratory equipment, the new facility is poised to serve R&D institutions both in the government and in the private sector in the coming months. Some of the laboratory equipment available at the PPMF includes a High and Ultra Pressure Liquid Chromatography System and a High-Resolution Mass Spectrometer, which are both used in identifying, quantifying, and purifying compounds.

 

We are proud of the PGC for the role that they play in advancing genomics and proteomics research in the country.” Usec. Guevara says. “It is our hope that with the inauguration of this facility, we will be able to provide a platform for collaborative work, and open new opportunities for our researchers to further grow their expertise in the field.”

 

In line with UP’s partnership with DOST, UP PGC executive director Dr. Cynthia P. Saloma mentioned in a separate interview that “DOST has always been a champion of our local researchers, by providing the mechanism (funding support) to make available highly technical scientific tools – for the advancement of scientific research in the country”.

 

Stay tuned for the launch of the PPMF webpage at the official website of the PGC (https://pgc.up.edu.ph/) for more information and updates.

 

ABOUT THE PHILIPPINE GENOME CENTER

The Philippine Genome Center is a genomics-focused multidisciplinary research unit that offers a full range of DNA sequencing services from single-gene sequencing to high-throughput sequencing and a suite of Bioinformatics services from scripting to full project workflows. With the addition of a new core facility devoted to protein analysis, PGC is able to offer a diverse range of Omics services in one roof. Under the Office of the Vice President for Academic Affairs of UP System, the Center’s main headquarters is located inside UP Diliman Campus. The Philippine Genome Center Building houses the DNA Sequencing Core Facility, Bioinformatics Core Facility, Core Facility for Biobanking, Clinical Genomics Laboratory, and the soon-to-launch Protein, Proteomics and Metabolomics Facility. 

 

In late 2019, the PGC’s Mindanao Satellite Facility, or PGC Mindanao, located inside UP Mindanao Campus had its ceremonial launch while the Visayas Satellite Facility commonly referred to as PGC Visayas, inaugurated its research and service laboratory in the last quarter of 2020.


Visit www.pgc.up.edu.ph to know more about the Philippine Genome Center and follow @phgenome on Twitter and Facebook for the latest news and activities related to genomics.

 

Source: https://www.pchrd.dost.gov.ph/news/6711-dost-up-to-inaugurate-cutting-edge-analytical-facility-for-proteomics-research 

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